Double- to Single-Strand Transition Induces Forces and Motion in DNA Origami Nanostructures.

ABSTRACT: The design of dynamic, reconfigurable devices is crucial for the bottom-up construction of artificial biological systems. DNA can be used as an engineering material for the de-novo design of such dynamic devices. A self-assembled DNA origami switch is presented that uses the transition from double- to single-stranded DNA and vice versa to create and annihilate an entropic force that drives a reversible conformational change inside the switch. It is distinctively demonstrated that a DNA single-strand that is extended with 0.34 nm per nucleotide - the extension this very strand has in the double-stranded configuration - exerts a contractive force on its ends leading to large-scale motion. The operation of this type of switch is demonstrated via transmission electron microscopy, DNA-PAINT sup

SUBMITTER: Gur FN 

PROVIDER: S-EPMC7611957 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature