Cell-surface SLC nucleoside transporters and purine levels modulate BRD4-dependent chromatin states.

ABSTRACT: Metabolism negotiates cell-endogenous requirements of energy, nutrients and building blocks with the immediate environment to enable various processes, including growth and differentiation. While there is an increasing number of examples of crosstalk between metabolism and chromatin, few involve uptake of exogenous metabolites. Solute carriers (SLCs) represent the largest group of transporters in the human genome and are responsible for the transport of a wide variety of substrates, including nutrients and metabolites. We aimed to investigate the possible involvement of SLC-mediated solutes uptake and cellular metabolism in regulating cellular epigenetic states. Here, we perform a CRISPR-Cas9 transporter-focused genetic screen and a metabolic compound library screen for the regulation of B

SUBMITTER: Li KC 

PROVIDER: S-EPMC7612075 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature