CD11c^-MHC2^low Macrophages Are a New Inflammatory and Dynamic Subset in Murine Adipose Tissue.

ABSTRACT:

Background

The prevalence of obesity is rising and leads to increased morbidity and mortality. Adipose tissue inflammation, due to accumulation and activation of adipose tissue macrophages (ATMs), is a key driver of this phenomenon. Macrophages are heterogeneous cells, adapting quickly to the microenvironment, resulting in so-called M1 or M2 macrophages. In this study, we describe the dynamics and inflammatory properties of a newly identified ATM subset in obese mice.

Methods

LDLR^-/- mice received a high fat diet (HFD) for 5 weeks or 16 weeks to induce obesity. Adipose tissues were isolated and immune cell subsets were analyzed with flow cytometry or microarray analysis. Bone marrow transplantation (BMT) using CD45.1 and CD45.2 LDLR^-/- mice was performe

SUBMITTER: Wetzels S 

PROVIDER: S-EPMC7612162 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature