Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET.

ABSTRACT: Cell division, aging, and stress recovery triggers spatial reorganization of cellular components in the cytoplasm, including membrane bound organelles, with molecular changes in their compositions and structures. However, it is not clear how these events are coordinated and how they integrate with regulation of molecular crowding. We use the budding yeast Saccharomyces cerevisiae as a model system to study these questions using recent progress in optical fluorescence microscopy and crowding sensing probe technology. We used a Förster Resonance Energy Transfer (FRET) based sensor, illuminated by confocal microscopy for high throughput analyses and Slimfield microscopy for single-molecule resolution, to quantify molecular crowding. We determine crowding in response to cellular growth of both

SUBMITTER: Lecinski S 

PROVIDER: S-EPMC7612257 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature