The functional proteome landscape of Escherichia coli.

ABSTRACT: Recent developments in high-throughput reverse genetics^1,2 have revolutionized our ability to map gene function and interactions^3-6. The power of these approaches depends on their ability to identify functionally associated genes, which elicit similar phenotypic changes across several perturbations (chemical, environmental or genetic) when knocked out^7-9. However, owing to the large number of perturbations, these approaches have been limited to growth or morphological readouts¹⁰. Here we use a high-content biochemical readout, thermal proteome profiling¹¹, to measure the proteome-wide protein abundance and thermal stability in response to 121 genetic perturbations in Escherichia coli. We show that thermal stability, and therefore the s

SUBMITTER: Mateus A 

PROVIDER: S-EPMC7612278 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature