Systematic characterization of human gut microbiome-secreted molecules by integrated multi-omics.

ABSTRACT: The human gut microbiome produces a complex mixture of biomolecules that interact with human physiology and play essential roles in health and disease. Crosstalk between micro-organisms and host cells is enabled by different direct contacts, but also by the export of molecules through secretion systems and extracellular vesicles. The resulting molecular network, comprised of various biomolecular moieties, has so far eluded systematic study. Here we present a methodological framework, optimized for the extraction of the microbiome-derived, extracellular biomolecular complement, including nucleic acids, (poly)peptides, and metabolites, from flash-frozen stool samples of healthy human individuals. Our method allows simultaneous isolation of individual biomolecular fractions from the same orig

SUBMITTER: De Saedeleer B 

PROVIDER: S-EPMC7612290 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature