Saccharomyces cerevisiae displays a stable transcription start site landscape in multiple conditions.

ABSTRACT: One of the fundamental processes that determine cellular fate is regulation of gene transcription. Understanding these regulatory processes is therefore essential for understanding cellular responses to changes in environmental conditions. At the core promoter, the regulatory region containing the transcription start site (TSS), all inputs regulating transcription are integrated. Here, we used Cap Analysis of Gene Expression (CAGE) to analyze the pattern of TSSs at four different environmental conditions (limited in ethanol, limited in nitrogen, limited in glucose and limited in glucose under anaerobic conditions) using the Saccharomyces cerevisiae strain CEN.PK113-7D. With this experimental setup, we were able to show that the TSS landscape in yeast is stable at different metabolic states

SUBMITTER: Borlin CS 

PROVIDER: S-EPMC7612425 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature