In-Droplet Electrophoretic Separation and Enrichment of Biomolecules.

ABSTRACT: We demonstrate the in-droplet separation and enrichment of molecules from small organic molecules to long nucleic acids (lambda DNA). Electric potentials are applied via two parallel three-dimensional electrodes, which interface the nanodroplets through polydimethylsiloxane (PDMS)-carbon composite membranes. These membranes enable the generation of uniform electric fields inside the droplets, while simultaneously preventing the formation of electrolytic byproducts. Biomolecules of different sizes migrate toward one side of the droplets, according to their net charge, when exposed to the electric field. Directly afterward, a Y-junction promotes droplet splitting, resulting in the generation of biomolecule-enriched daughter droplets. Biomolecules were fluorescently labeled, and fluorescence

SUBMITTER: Saucedo-Espinosa MA 

PROVIDER: S-EPMC7612497 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature