<i>Contour</i>, a semi-automated segmentation and quantitation tool for cryo-soft-X-ray tomography.

ABSTRACT: Cryo-soft-X-ray tomography is being increasingly used in biological research to study the morphology of cellular compartments and how they change in response to different stimuli, such as viral infections. Segmentation of these compartments is limited by time-consuming manual tools or machine learning algorithms that require extensive time and effort to train. Here we describe Contour, a new, easy-to-use, highly automated segmentation tool that enables accelerated segmentation of tomograms to delineate distinct cellular compartments. Using Contour, cellular structures can be segmented based on their projection intensity and geometrical width by applying a threshold range to the image and excluding noise smaller in width than the cellular compartments of interest. This method

SUBMITTER: Nahas KL 

PROVIDER: S-EPMC7612748 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature