Transcriptional cofactors display specificity for distinct types of core promoters.

ABSTRACT: Transcriptional cofactors (COFs) communicate regulatory cues from enhancers to promoters and are central effectors of transcription activation and gene expression¹. Although some COFs have been shown to prefer certain promoter types^2-5 over others (for example, see refs ^6,7), the extent to which different COFs display intrinsic specificities for distinct promoters is unclear. Here we use a high-throughput promoter-activity assay in Drosophila melanogaster S2 cells to screen 23 COFs for their ability to activate 72,000 candidate core promoters (CPs). We observe differential activation of CPs, indicating distinct regulatory preferences or 'compatibilities'^8,9 between COFs and specific types of CPs. These functionally distinct CP types are different

SUBMITTER: Haberle V 

PROVIDER: S-EPMC7613045 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature