Differential cofactor dependencies define distinct types of human enhancers.

ABSTRACT: All multicellular organisms rely on differential gene transcription regulated by genomic enhancers, which function through cofactors that are recruited by transcription factors^1,2. Emerging evidence suggests that not all cofactors are required at all enhancers^3-5, yet whether these observations reflect more general principles or distinct types of enhancers remained unknown. Here we categorized human enhancers by their cofactor dependencies and show that these categories provide a framework to understand the sequence and chromatin diversity of enhancers and their roles in different gene-regulatory programmes. We quantified enhancer activities along the entire human genome using STARR-seq⁶ in HCT116 cells, following the rapid degradation of eight cofactors. T

SUBMITTER: Neumayr C 

PROVIDER: S-EPMC7613064 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature