Target site selection and remodelling by type V CRISPR-transposon systems.

ABSTRACT: Canonical CRISPR-Cas systems provide adaptive immunity against mobile genetic elements¹. However, type I-F, I-B and V-K systems have been adopted by Tn7-like transposons to direct RNA-guided transposon insertion^2-7. Type V-K CRISPR-associated transposons rely on the pseudonuclease Cas12k, the transposase TnsB, the AAA+ ATPase TnsC and the zinc-finger protein TniQ⁷, but the molecular mechanism of RNA-directed DNA transposition has remained elusive. Here we report cryo-electron microscopic structures of a Cas12k-guide RNA-target DNA complex and a DNA-bound, polymeric TnsC filament from the CRISPR-associated transposon system of the photosynthetic cyanobacterium Scytonema hofmanni. The Cas12k complex structure reveals an intricate guide RNA architecture and cr

SUBMITTER: Querques I 

PROVIDER: S-EPMC7613401 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature