A novel translational model for fetoscopic intratracheal delivery of nanoparticles in piglets.

ABSTRACT:

Objectives

The aim of this study was to assess the feasibility of fetal tracheal injection in the late-gestational pig to target the airways.

Methods

Following laparotomy and hysterotomy, fetoscopy was performed in pregnant sows to access the fetal trachea. Two volumes of fluospheres were injected (1 and 3 mL). Fluosphere distribution to the different lung lobes was investigated by microscopy. Possible fetal airway injury, caused by the surgical procedure or intratracheal injection, was investigated. Lung morphology and fetal lung volumes were calculated by micro computed tomography (μCT).

Results

Intratracheal administration was successfully performed in 20/21 fetuses. Analysis by confocal microscopy demonstrated that 3 mL, and not 1 mL, most efficiently targeted all

SUBMITTER: Carlon MS 

PROVIDER: S-EPMC7613458 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature