Transcriptional neighborhoods regulate transcript isoform lengths and expression levels.

ABSTRACT: Sequence features of genes and their flanking regulatory regions are determinants of RNA transcript isoform expression and have been used as context-independent plug-and-play modules in synthetic biology. However, genetic context-including the adjacent transcriptional environment-also influences transcript isoform expression levels and boundaries. We used synthetic yeast strains with stochastically repositioned genes to systematically disentangle the effects of sequence and context. Profiling 120 million full-length transcript molecules across 612 genomic perturbations, we observed sequence-independent alterations to gene expression levels and transcript isoform boundaries that were influenced by neighboring transcription. We identified features of transcriptional context that could predic

SUBMITTER: Brooks AN 

PROVIDER: S-EPMC7613581 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature