Engineering Ligand and Receptor Pairs with LIPSTIC to Track Cell-Cell Interactions.

ABSTRACT: Interactions between different cell types are critical for a plethora of biological processes, such as the immune response. We recently developed a novel technology, called LIPSTIC (labeling of immune partnership by SorTagging intercellular contacts), that allows for identifying cells undergoing specific interactions thanks to an enzymatic labeling reaction. Our work demonstrated the use of this technology to monitor interactions between immune cells, both in vitro and in vivo, by the genetic engineering of CD40 and CD40L, an essential costimulatory axis between antigen-presenting cells and T cells. Here we describe protocols to design novel LIPSTIC-engineered ligand and receptor pairs, clone constructs into retroviral expression vector, perform their initial validation, and use them to me

SUBMITTER: Alberti D 

PROVIDER: S-EPMC7613713 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature