Ontology highlight
ABSTRACT:
SUBMITTER: Nahmad AD
PROVIDER: S-EPMC7613940 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Nahmad Alessio David AD Reuveni Eli E Goldschmidt Ella E Tenne Tamar T Liberman Meytal M Horovitz-Fried Miriam M Khosravi Rami R Kobo Hila H Reinstein Eyal E Madi Asaf A Ben-David Uri U Barzel Adi A
Nature biotechnology 20220630 12
Multiple clinical trials of allogeneic T cell therapy use site-specific nucleases to disrupt T cell receptor (TCR) and other genes<sup>1-6</sup>. In this study, using single-cell RNA sequencing, we investigated genome editing outcomes in primary human T cells transfected with CRISPR-Cas9 and guide RNAs targeting genes for TCR chains and programmed cell death protein 1. Four days after transfection, we found a loss of chromosome 14, harboring the TCRα locus, in up to 9% of the cells and a chromos ...[more]