Spatial profiling of early primate gastrulation in utero.

ABSTRACT: Gastrulation controls the emergence of cellular diversity and axis patterning in the early embryo. In mammals, this transformation is orchestrated by dynamic signalling centres at the interface of embryonic and extraembryonic tissues^1-3. Elucidating the molecular framework of axis formation in vivo is fundamental for our understanding of human development^4-6 and to advance stem-cell-based regenerative approaches⁷. Here we illuminate early gastrulation of marmoset embryos in utero using spatial transcriptomics and stem-cell-based embryo models. Gaussian process regression-based 3D transcriptomes delineate the emergence of the anterior visceral endoderm, which is hallmarked by conserved (HHEX, LEFTY2, LHX1) and primate-specific (POSTN, SDC4, FZD5) factors. WN

SUBMITTER: Bergmann S 

PROVIDER: S-EPMC7614364 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature