Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation.

ABSTRACT: The early specification and rapid growth of extraembryonic membranes are distinctive hallmarks of primate embryogenesis. These complex tasks are resolved through an intricate combination of signals controlling the induction of extraembryonic lineages and, at the same time, safeguarding the pluripotent epiblast. Here, we delineate the signals orchestrating primate epiblast and amnion identity. We encapsulated marmoset pluripotent stem cells into agarose microgels and identified culture conditions for the development of epiblast- and amnion-spheroids. Spatial identity mapping authenticated spheroids generated in vitro by comparison with marmoset embryos in vivo. We leveraged the microgel system to functionally interrogate the signalling environment of the post-implantation primate embryo. Si

SUBMITTER: Munger C 

PROVIDER: S-EPMC7614365 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature