Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology.

ABSTRACT: Ex vivo preparations enable the study of many neurophysiological processes in isolation from the rest of the body while preserving local tissue structure. This work describes the preparation of rat sciatic nerves for ex vivo neurophysiology, including buffer preparation, animal procedures, equipment setup and neurophysiological recording. This work provides an overview of the different types of experiments possible with this method. The outlined method aims to provide 6 h of stimulation and recording on extracted peripheral nerve tissue in tightly controlled conditions for optimal consistency in results. Results obtained using this method are A-fibre compound action potentials (CAP) with peak-to-peak amplitudes in the millivolt range over the entire duration of the experiment. CAP amplitud

SUBMITTER: Rapeaux A 

PROVIDER: S-EPMC7614932 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature