Large scale microfluidic CRISPR screening for increased amylase secretion in yeast.

ABSTRACT: Key to our ability to increase recombinant protein production through secretion is a better understanding of the pathways that interact to translate, process and export mature proteins to the surrounding environment, including the supporting cellular machinery that supplies necessary energy and building blocks. By combining droplet microfluidic screening with large-scale CRISPR libraries that perturb the expression of the majority of coding and non-coding genes in S. cerevisiae, we identified 345 genes for which an increase or decrease in gene expression resulted in increased secretion of α-amylase. Our results show that modulating the expression of genes involved in the trafficking of vesicles, endosome to Golgi transport, the phagophore assembly site, the cell cycle and energy sup

SUBMITTER: Johansson SA 

PROVIDER: S-EPMC7614956 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature