ADAMTS-7 Modulates Atherosclerotic Plaque Formation by Degradation of TIMP-1.

ABSTRACT:

Background

The ADAMTS7 locus was genome-wide significantly associated with coronary artery disease. Lack of the ECM (extracellular matrix) protease ADAMTS-7 (A disintegrin and metalloproteinase-7) was shown to reduce atherosclerotic plaque formation. Here, we sought to identify molecular mechanisms and downstream targets of ADAMTS-7 mediating the risk of atherosclerosis.

Methods

Targets of ADAMTS-7 were identified by high-resolution mass spectrometry of atherosclerotic plaques from Apoe^-/- and Apoe^-/-Adamts7^-/- mice. ECM proteins were identified using solubility profiling. Putative targets were validated using immunofluorescence, in vitro degradation assays, coimmunoprecipitation, and Förster resonance energy transfer-based protein-pro

SUBMITTER: Sharifi MA 

PROVIDER: S-EPMC7615141 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature