Multi-Knock-a multi-targeted genome-scale CRISPR toolbox to overcome functional redundancy in plants.

ABSTRACT: Plant genomes are characterized by large and complex gene families that often result in similar and partially overlapping functions. This genetic redundancy severely hampers current efforts to uncover novel phenotypes, delaying basic genetic research and breeding programmes. Here we describe the development and validation of Multi-Knock, a genome-scale clustered regularly interspaced short palindromic repeat toolbox that overcomes functional redundancy in Arabidopsis by simultaneously targeting multiple gene-family members, thus identifying genetically hidden components. We computationally designed 59,129 optimal single-guide RNAs that each target two to ten genes within a family at once. Furthermore, partitioning the library into ten sublibraries directed towards a different functional gr

SUBMITTER: Hu Y 

PROVIDER: S-EPMC7615256 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature