The chicken chorioallantoic membrane as a low-cost, high-throughput model for cancer imaging.

ABSTRACT: Mouse models are invaluable tools for radiotracer development and validation. They are, however, expensive, low throughput, and are constrained by animal welfare considerations. Here, we assessed the chicken chorioallantoic membrane (CAM) as an alternative to mice for preclinical cancer imaging studies. NCI-H460 FLuc cells grown in Matrigel on the CAM formed vascularized tumors of reproducible size without compromising embryo viability. By designing a simple method for vessel cannulation it was possible to perform dynamic PET imaging in ovo, producing high tumor-to-background signal for both ¹⁸F-2-fluoro-2-deoxy-D-glucose (¹⁸F-FDG) and (4S)-4-(3-¹⁸F-fluoropropyl)-L-glutamate (¹⁸F-FSPG). The pattern of ¹⁸F-FDG tumor uptake were similar

SUBMITTER: Smith LM 

PROVIDER: S-EPMC7615542 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature