Intrafemoral Injection of Human Hematopoietic Stem and Progenitor Cells into Immunocompromised Mice.

ABSTRACT: Hematopoietic stem cells (HSCs) are defined by their lifelong ability to produce all blood cell types. This is operationally tested by transplanting cell populations containing HSCs into syngeneic or immunocompromised mice. The size and multilineage composition of the graft is then measured over time, usually by flow cytometry. Classically, a population containing HSCs is injected into the circulation of the animal, after which the HSCs home to the bone marrow, where they lodge and begin blood production. Alternatively, HSCs and/or progenitor cells (HSPCs) can be placed directly in the bone marrow cavity. This paper describes a protocol for intrafemoral injection of human HSPCs into immunodeficient mice. In short, preconditioned mice are anesthetized, and a small hole is drilled through th

SUBMITTER: Calderbank EF 

PROVIDER: S-EPMC7615601 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature