Studying micro to millisecond protein dynamics using simple amide ¹⁵N CEST experiments supplemented with major-state R₂ and visible peak-position constraints.

ABSTRACT: Over the last decade amide ¹⁵N CEST experiments have emerged as a popular tool to study protein dynamics that involves exchange between a 'visible' major state and sparsely populated 'invisible' minor states. Although initially introduced to study exchange between states that are in slow exchange with each other (typical exchange rates of, 10 to 400 s^-1), they are now used to study interconversion between states on the intermediate to fast exchange timescale while still using low to moderate (5 to 350 Hz) 'saturating' B₁ fields. The ¹⁵N CEST experiment is very sensitive to exchange as the exchange delay T_EX can be quite long (~0.5 s) allowing for a large number of exchange events to occur making it a very powerful tool to detect minor

SUBMITTER: Khandave NP 

PROVIDER: S-EPMC7615914 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature