Seed Imbibition and Metabolism Contribute Differentially to Initial Assembly of the Soybean Holobiont.

ABSTRACT: Seed germination critically determines successful plant establishment and agricultural productivity. In the plant holobiont's life cycle, seeds are hubs for microbial communities' assembly, but what exactly shapes the holobiont during germination remains unknown. Here, 16S rRNA gene amplicon sequencing characterized the bacterial communities in embryonic compartments (cotyledons and axes) and on seed coats pre- and post-germination of four soybean (Glycine max) cultivars, in the presence or absence of exogenous abscisic acid (ABA), which prevented germination and associated metabolism of seeds that had imbibed. Embryonic compartments were metabolically profiled during germination to design minimal media mimicking the seed endosphere for bacterial growth assays. The distinction betwe

SUBMITTER: Gerna D 

PROVIDER: S-EPMC7616048 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature