Opportunities and challenges in design and optimization of protein function.

ABSTRACT: The field of protein design has made remarkable progress over the past decade. Historically, the low reliability of purely structure-based design methods limited their application, but recent strategies that combine structure-based and sequence-based calculations, as well as machine learning tools, have dramatically improved protein engineering and design. In this Review, we discuss how these methods have enabled the design of increasingly complex structures and therapeutically relevant activities. Additionally, protein optimization methods have improved the stability and activity of complex eukaryotic proteins. Thanks to their increased reliability, computational design methods have been applied to improve therapeutics and enzymes for green chemistry and have generated vaccine antigens, a

SUBMITTER: Listov D 

PROVIDER: S-EPMC7616297 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature