A human embryonic limb cell atlas resolved in space and time.

ABSTRACT: Human limbs emerge during the fourth post-conception week as mesenchymal buds, which develop into fully formed limbs over the subsequent months¹. This process is orchestrated by numerous temporally and spatially restricted gene expression programmes, making congenital alterations in phenotype common². Decades of work with model organisms have defined the fundamental mechanisms underlying vertebrate limb development, but an in-depth characterization of this process in humans has yet to be performed. Here we detail human embryonic limb development across space and time using single-cell and spatial transcriptomics. We demonstrate extensive diversification of cells from a few multipotent progenitors to myriad differentiated cell states, including several novel cell popul

SUBMITTER: Zhang B 

PROVIDER: S-EPMC7616500 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature