Reconstitution of Membrane-tethered Minimal Actin Cortices on Supported Lipid Bilayers.

ABSTRACT: The surface of a living cell provides a versatile active platform for numerous cellular processes, which arise from the interplay of the plasma membrane with the underlying actin cortex. In the past decades, reconstituted, minimal systems based on supported lipid bilayers in combination with actin filament networks have proven to be very instrumental in unraveling basic mechanisms and consequences of membrane-tethered actin networks, as well as in studying the functions of individual membrane-associated proteins. Here, we describe how to reconstitute such active composite systems in vitro that consist of fluid supported lipid bilayers coupled via membrane-associated actin-binding proteins to dynamic actin filaments and myosin motors that can be readily observed via total internal reflectio

SUBMITTER: Koster DV 

PROVIDER: S-EPMC7616522 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature