Viral vectors expressing group B meningococcal outer membrane proteins induce strong antibody responses but fail to induce functional bactericidal activity.

ABSTRACT:

Objective

Adenoviral vectored vaccines, with the appropriate gene insert, induce cellular and antibody responses against viruses, parasites and intracellular pathogens such as Mycobacterium tuberculosis. Here we explored their capacity to induce functional antibody responses to meningococcal transmembrane outer membrane proteins.

Methods

Vectors expressing porin A and ferric enterobactin receptor A antigens were generated, and their immunogenicity assessed in mice using binding and bactericidal assays.

Results

The viral vectors expressed the bacterial proteins in an in vitro cell-infection assay and, after immunisation of mice, induced higher titres (>10⁵ end-point titre) and longer lasting (>32 weeks) transgene-specific antibody responses in vivo than did

SUBMITTER: Marsay L 

PROVIDER: S-EPMC7616632 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature