A global analysis of transcription reveals two modes of Spt4/5 recruitment to archaeal RNA polymerase.

ABSTRACT: The archaeal transcription apparatus is closely related to the eukaryotic RNA polymerase (RNAP) II system, while archaeal genomes are more similar to bacteria with densely packed genes organized in operons. This makes understanding transcription in archaea vital, both in terms of molecular mechanisms and evolution. Very little is known about how archaeal cells orchestrate transcription on a systems level. We have characterized the genome-wide occupancy of the Methanocaldococcus jannaschii transcription machinery and its transcriptome. Our data reveal how the TATA and BRE promoter elements facilitate recruitment of the essential initiation factors TATA-binding protein and transcription factor B, respectively, which in turn are responsible for the loading of RNAP into the transcription units

SUBMITTER: Smollett K 

PROVIDER: S-EPMC7616672 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature