Mechanism for the initiation of spliceosome disassembly.

ABSTRACT: Precursor-mRNA (pre-mRNA) splicing requires the assembly, remodelling and disassembly of the multi-megadalton ribonucleoprotein complex called the spliceosome¹. Recent studies have shed light on spliceosome assembly and remodelling for catalysis^2-6, but the mechanism of disassembly remains unclear. Here we report cryo-electron microscopy structures of nematode and human terminal intron lariat spliceosomes along with biochemical and genetic data. Our results uncover how four disassembly factors and the conserved RNA helicase DHX15 initiate spliceosome disassembly. The disassembly factors probe large inner and outer spliceosome surfaces to detect the release of ligated mRNA. Two of these factors, TFIP11 and C19L1, and three general spliceosome subunits, SYF1, SYF2 and S

SUBMITTER: Vorlander MK 

PROVIDER: S-EPMC7616679 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature