Single-cell Sequencing of Circulating Human Plasmablasts during Staphylococcus aureus Bacteremia.

ABSTRACT: Staphylococcus aureus is the major cause of healthcare-associated infections, including life-threatening conditions as bacteremia, endocarditis, and implant-associated infections. Despite adequate antibiotic treatment, the mortality of S. aureus bacteremia remains high. This calls for different strategies to treat this infection. In past years, sequencing of Ab repertoires from individuals previously exposed to a pathogen emerged as a successful method to discover novel therapeutic monoclonal Abs and understand circulating B cell diversity during infection. In this paper, we collected peripheral blood from 17 S. aureus bacteremia patients to study circulating plasmablast responses. Using single-cell transcriptome gene expression combined with sequencing of variable heavy and light Ig genes

SUBMITTER: Kerkman PF 

PROVIDER: S-EPMC7616744 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature