Functional Coiled-Coil-like Assembly by Knob-into-Hole Packing of Single Heptad Repeat.

ABSTRACT: Coiled-coil peptides represent the principal building blocks for structure-based design of bionanomaterials. The sequence-structure relationship and precise nanoscale ordering of the coiled-coil helices originate from the knob-into-hole (KIH) packing of side chains. The helical interface stabilized by the KIH interaction is known to have chain lengths ranging from 30 to 1000 residues. Yet the shortest peptide required for oligomerization through KIH assembly is still unknown. Here, we report that through atomic resolution a minimal seven-residue amphipathic helix forms a different type of KIH motif, termed "supramolecular KIH packing", which confers an exceptional stability to the helical dimers. Significantly, at a low pH, the peptide self-assembles into nanofibers with coiled-coil archit

SUBMITTER: Mondal S 

PROVIDER: S-EPMC7616943 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature