Microfibrous Scaffolds Guide Stem Cell Lumenogenesis and Brain Organoid Engineering.

ABSTRACT: 3D organoids are widely used as tractable in vitro models capable of elucidating aspects of human development and disease. However, the manual and low-throughput culture methods, coupled with a low reproducibility and geometric heterogeneity, restrict the scope and application of organoid research. Combining expertise from stem cell biology and bioengineering offers a promising approach to address some of these limitations. Here, melt electrospinning writing is used to generate tuneable grid scaffolds that can guide the self-organization of pluripotent stem cells into patterned arrays of embryoid bodies. Grid geometry is shown to be a key determinant of stem cell self-organization, guiding the position and size of emerging lumens via curvature-controlled tissue growth. Two distinct methods

SUBMITTER: Ritzau-Reid KI 

PROVIDER: S-EPMC7617127 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature