Generating human blastoids modeling blastocyst-stage embryos and implantation.

ABSTRACT: Human early development sets the stage for embryonic and adult life but remains difficult to investigate. A solution came from the ability of stem cells to organize into structures resembling preimplantation embryos-blastocysts-that we termed blastoids. This embryo model is available in unlimited numbers and could thus support scientific and medical advances. However, its predictive power depends on how faithfully it recapitulates the blastocyst. Here, we describe how we formed human blastoids that (1) efficiently achieve the morphology of the blastocyst and (2) form lineages according to the pace and sequence of blastocyst development, (3) ultimately forming cells that transcriptionally reflect the blastocyst (preimplantation stage). We employ three different commercially available 96- an

SUBMITTER: Heidari Khoei H 

PROVIDER: S-EPMC7617227 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature