Haematococcus lacustris genome assembly and annotation reveal diploid genetic traits and stress-induced gene expression patterns.

ABSTRACT: The green alga Haematococcus lacustris (formerly Haematococcus pluvialis) is a primary source of astaxanthin, a ketocarotenoid with high antioxidant activity and several industrial applications. Here, the Haematococcus lacustris highly repetitive genome was reconstructed by exploiting next-generation sequencing integrated with Hi-C scaffolding, obtaining a 151 Mb genome assembly in 32 scaffolds at a near-chromosome level with high continuity. Surprisingly, the distribution of the single-nucleotide-polymorphisms identified demonstrates a diploid configuration for the Haematococcus genome, further validated by Sanger sequencing of heterozygous regions. Functional annotation and RNA-seq data enabled the identification of 13,946 nuclear genes, with >5000 genes not p

SUBMITTER: Marcolungo L 

PROVIDER: S-EPMC7617258 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature