Deconvolution of Gene Expression Noise into Spatial Dynamics of Transcription Factor-Promoter Interplay.

ABSTRACT: Gene expression noise is not only the mere consequence of stochasticity, but also a signal that reflects the upstream physical dynamics of the cognate molecular machinery. Soil bacteria facing recalcitrant pollutants exploit noise of catabolic promoters to deploy beneficial phenotypes such as metabolic bet-hedging and/or division of biochemical labor. Although the role of upstream promoter-regulator interplay in the origin of this noise is little understood, its specifications are probably ciphered in flow cytometry data patterns. We studied Pm promoter activity of the environmental bacterium Pseudomonas putida and its cognate regulator XylS by following expression of Pm-gfp fusions in single cells. Using mathematical modeling and computational simulations, we determined the kinetic proper

SUBMITTER: Goni-Moreno A 

PROVIDER: S-EPMC7617343 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature