Live tracking of replisomes reveals nutrient-dependent regulation of replication elongation rates in Caulobacter crescentus.

ABSTRACT: In bacteria, commitment to genome replication (initiation) is intricately linked to nutrient availability. Whether growth conditions affect other stages of replication beyond initiation remains to be systematically studied. To address this, we assess the replication dynamics of Caulobacter crescentus, a bacterium that undergoes only a single round of replication per cell cycle, by tracking the replisome across various growth phases and nutrient conditions. We find that the replication elongation rates slow down as cells transition from exponential (high-nutrient) to stationary (low-nutrient) phase, and this contributes significantly to the overall cell-cycle delay. Although elongation rates are correlated with growth rates, both properties are differentially influenced by nutrient status.

SUBMITTER: Adhikashreni IS 

PROVIDER: S-EPMC7617702 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature