A Self-Renewing Biomimetic Skeletal Muscle Construct Engineered using Induced Myogenic Progenitor Cells.

ABSTRACT: Skeletal muscle represents a highly organized tissue that primarily regenerates by myogenic stem cells. Mimicking an in vitro skeletal muscle differentiation program that contains self-renewing muscle stem cells and aligned myotubes is considered challenging. This study presents the engineering of a biomimetic muscle construct that can self-regenerate and produce aligned myotubes using induced myogenic progenitor cells (iMPCs), a heterogeneous culture consisting of skeletal muscle stem, progenitor, and differentiated cells. Utilizing electrospinning, polycaprolactone (PCL) substrates are fabricated to facilitate iMPC-differentiation into aligned myotubes by controlling PCL fiber orientation. Newly-conceived constructs contain organized multinucleated myotubes alongside self-renewing stem c

SUBMITTER: Kim I 

PROVIDER: S-EPMC7617902 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature