A 3D-printed flow-cell for on-grid purification of electron microscopy samples directly from lysate.

ABSTRACT: While recent advances in cryo-EM, coupled with single particle analysis, have the potential to allow structure determination in a near-native state from vanishingly few individual particles, this vision has yet to be realised in practise. Requirements for particle numbers that currently far exceed the theoretical lower limits, challenges with the practicalities of achieving high concentrations for difficult-to-produce samples, and inadequate sample-dependent imaging conditions, all result in significant bottlenecks preventing routine structure determination using cryo-EM. Therefore, considerable efforts are being made to circumvent these bottlenecks by developing affinity purification of samples on-grid; at once obviating the need to produce large amounts of protein, as well as more direct

SUBMITTER: Ramlaul K 

PROVIDER: S-EPMC7618051 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature