Heterologous expression of the cyanobacterial fructose-1,6-/sedoheptulose-1,7-bisphosphatase in <i>Chlamydomonas reinhardtii</i> causes increased cell size and biomass productivity in mixotrophic conditions.

ABSTRACT: The Calvin-Benson-Bassham (CBB) cycle is the metabolic pathway responsible for CO₂ assimilation in oxygenic photosynthetic organisms. Two key rate-limiting steps in this cycle are catalyzed by the enzymes fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase), making them promising targets for genetic enhancement to improve carbon fixation. A potential strategy involves overexpressing a cyanobacterial dual-function FBP/SBPase, which catalyzes both reactions. Overexpression of this enzyme in tobacco plants or in other plants led to an increase in growth rate and biomass accumulation. Here, the overexpression of the same enzyme was achieved in Chlamydomonas reinhardtii. The recombinant cyanobacterial FBP/SBPase isolated from C. reinhardtii

SUBMITTER: Bussola M 

PROVIDER: S-EPMC7618607 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature