Direct recruitment of Mis18 to interphase spindle pole bodies promotes CENP-A chromatin assembly.

ABSTRACT: CENP-A chromatin specifies mammalian centromere identity, and its chaperone HJURP replenishes CENP-A when recruited by the Mis18 complex (Mis18C) via M18BP1/KNL2 to CENP-C at kinetochores during interphase. However, the Mis18C recruitment mechanism remains unresolved in species lacking M18BP1, such as fission yeast. Fission yeast centromeres cluster at G2 spindle pole bodies (SPBs) when CENP-A^Cnp1 is replenished and where Mis18C also localizes. We show that SPBs play an unexpected role in concentrating Mis18C near centromeres through the recruitment of Mis18 by direct binding to the major SPB linker of nucleoskeleton and cytoskeleton (LINC) component Sad1. Mis18C recruitment by Sad1 is important for CENP-A^Cnp1 chromatin establishment and acts in parallel with a CENP-C

SUBMITTER: London N 

PROVIDER: S-EPMC7618776 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature