Theabrownin inhibits the cytoskeleton‑dependent cell cycle, migration and invasion of human osteosarcoma cells through NF‑κB pathway‑related mechanisms.

ABSTRACT: Considering the high metastatic potential of osteosarcoma, not only pro‑apoptosis, but also anti‑metastasis is important for anti‑osteosarcoma therapy. Previously, the authors reported the pro‑apoptotic and tumor‑inhibitory effects of theabrownin (TB) on osteosarcoma cells; however, its effects on the metastasis‑related migration and invasion of osteosarcoma cells remain unknown. The present study conducted RNA sequencing (RNA‑seq) on xenograft zebrafish samples and performed in vitro experiments, including RT‑qPCR, cell viability analysis, clone formation assay, cell cycle analysis, immunofluorescence, cell migration assay, cell invasion assay, wound healing assay and western blot (WB) analysis to evaluate the anti‑metastatic effects and mechanism of TB against osteosarcoma cells. The RNA

SUBMITTER: Jin W 

PROVIDER: S-EPMC7640368 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature