In vivo and in vitro inflammatory responses to fine particulate matter (PM_2.5) from China and California.

ABSTRACT: Ambient PM_2.5 was collected during the winter season from Taiyuan, Shanxi, China; Jinan, Shandong, China; and Sacramento, California, USA, and used to create PM_SX, PM_SD, and PM_CA extracts, respectively. Time-lag experiments were performed to explore the in vivo and in vitro toxicity of the PM extracts. In vivo inflammatory lung responses were assessed in BALB/c mice using a single oropharyngeal aspiration (OPA) of PM extract or vehicle (CTRL) on Day 0. Necropsies were performed on Days 1, 2, and 4 post-OPA, and pulmonary effects were determined using bronchoalveolar lavage (BAL) and histopathology. On Day 1, BAL neutrophils were significantly elevated in all PM- versus CTRL-exposed mice, with PM_CA producing the strongest response. Howe

SUBMITTER: Yuan W 

PROVIDER: S-EPMC7641014 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature