Microanatomy of the Human Atherosclerotic Plaque by Single-Cell Transcriptomics.

ABSTRACT:

Rationale

Atherosclerotic lesions are known for their cellular heterogeneity, yet the molecular complexity within the cells of human plaques has not been fully assessed.

Objective

Using single-cell transcriptomics and chromatin accessibility, we gained a better understanding of the pathophysiology underlying human atherosclerosis.

Methods and results

We performed single-cell RNA and single-cell ATAC sequencing on human carotid atherosclerotic plaques to define the cells at play and determine their transcriptomic and epigenomic characteristics. We identified 14 distinct cell populations including endothelial cells, smooth muscle cells, mast cells, B cells, myeloid cells, and T cells and identified multiple cellular activation states and suggested cellular interconversi

SUBMITTER: Depuydt MAC 

PROVIDER: S-EPMC7641189 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature