The structural ensemble of a Holliday junction determined by X-ray scattering interference.

ABSTRACT: The DNA four-way (Holliday) junction is the central intermediate of genetic recombination, yet key aspects of its conformational and thermodynamic properties remain unclear. While multiple experimental approaches have been used to characterize the canonical X-shape conformers under specific ionic conditions, the complete conformational ensemble of this motif, especially at low ionic conditions, remains largely undetermined. In line with previous studies, our single-molecule Förster resonance energy transfer (smFRET) measurements of junction dynamics revealed transitions between two states under high salt conditions, but smFRET could not determine whether there are fast and unresolvable transitions between distinct conformations or a broad ensemble of related states under low and intermedia

SUBMITTER: Zettl T 

PROVIDER: S-EPMC7641307 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature