A low-bias and sensitive small RNA library preparation method using randomized splint ligation.

ABSTRACT: Small RNAs are important regulators of gene expression and are involved in human development and disease. Next generation sequencing (NGS) allows for scalable, genome-wide studies of small RNA; however, current methods are challenged by low sensitivity and high bias, limiting their ability to capture an accurate representation of the cellular small RNA population. Several studies have shown that this bias primarily arises during the ligation of single-strand adapters during library preparation, and that this ligation bias is magnified by 2'-O-methyl modifications (2'OMe) on the 3' terminal nucleotide. In this study, we developed a novel library preparation process using randomized splint ligation with a cleavable adapter, a design which resolves previous challenges associated with this lig

SUBMITTER: Maguire S 

PROVIDER: S-EPMC7641310 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature