Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility.

ABSTRACT:

Purpose

Secreted protein acidic and rich in cysteine (SPARC) is a matricellular protein that regulates intraocular pressure (IOP) by altering extracellular matrix (ECM) homeostasis within the trabecular meshwork (TM). We hypothesized that the lower IOP previously observed in SPARC -/- mice is due to a greater outflow facility.

Methods

Mouse outflow facility (Clive) was determined by multiple flow rate infusion, and episcleral venous pressure (Pe) was estimated by manometry. The animals were then euthanized, eliminating aqueous formation rate (Fin) and Pe. The C value was determined again (Cdead) while Fin was reduced to zero. Additional mice were euthanized for immunohistochemistry to analyze ECM components of the TM.

Results

The Clive and Cdead of SPARC -/- mice were

SUBMITTER: Yu L 

PROVIDER: S-EPMC7641442 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature