Computational model demonstrates that Ndc80-associated proteins strengthen kinetochore-microtubule attachments in metaphase.

ABSTRACT: Chromosome segregation is mediated by spindle microtubules that attach to the kinetochore via dynamic protein complexes, such as Ndc80, Ska, Cdt1 and ch-TOG during mitotic metaphase. While experimental studies have previously shown that these proteins and protein complexes are all essential for maintaining a stable kinetochore-microtubule (kMT) interface, their exact roles in the mitotic metaphase remains elusive. In this study, we employed experimental and computational methods in order to characterize how these proteins can strengthen kMT attachments in both nonload-bearing and load-bearing conditions, typical of prometaphase and metaphase, respectively. Immunofluorescence staining of HeLa cells showed that the levels of Ska and Cdt1 significantly increased from prometaphase to metaphase

SUBMITTER: Campbell S 

PROVIDER: S-EPMC7641512 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature